Ultrasensitive electrochemical immunosensor employing glucose oxidase catalyzed deposition of gold nanoparticles for signal amplification.
Identifieur interne : 001058 ( Main/Exploration ); précédent : 001057; suivant : 001059Ultrasensitive electrochemical immunosensor employing glucose oxidase catalyzed deposition of gold nanoparticles for signal amplification.
Auteurs : RBID : pubmed:21782410English descriptors
- KwdEn :
- MESH :
- chemical , analysis : Immunoglobulin G.
- chemical , chemistry : Enzymes, Immobilized, Glucose Oxidase, Gold.
- chemistry : Metal Nanoparticles.
- Animals, Biosensing Techniques, Electrochemical Techniques, Electrodes, Immunoassay, Mice, Tin Compounds.
Abstract
This paper describes a novel enzymatic amplification strategy for ultrasensitive electrochemical immunosensing. This approach utilizes glucose oxidase for the enzymatic deposition of gold nanoparticles onto an indium tin oxide (ITO) electrode surface using a novel gold developer solution consisting of 20 mM of glucose, 20 mM of NaSCN, 0.5 M of p-benzoquinone (PBQ) and 1 mM of AuCl(4)(-) dissolved in 0.1 M of pH 7.5 phosphate buffer solution. The amount of gold deposited was quantified electrochemically by monitoring the reduction of gold oxide in an aqueous solution of 0.5 M of H(2)SO(4), which was correlated to the amount of antigens in the solution. The effectiveness of this strategy was demonstrated experimentally through the construction of an immunosensor for the detection of mouse IgG using a sandwich immunoassay in a linear dynamic range of 5 pg/ml to 50 ng/ml. A good mean apparent recovery in the range of 88-102% was obtained over the entire linear dynamic range of the sensor response in the serum samples. This suggested that the immunosensor would be useful for the testing of proteins in real clinical samples.
DOI: 10.1016/j.bios.2011.06.007
PubMed: 21782410
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<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Ultrasensitive electrochemical immunosensor employing glucose oxidase catalyzed deposition of gold nanoparticles for signal amplification.</title><author><name sortKey="Zhang, Jie" uniqKey="Zhang J">Jie Zhang</name><affiliation wicri:level="1"><nlm:affiliation>Institute of Bioengineering and Nanotechnology, The Nanos, Singapore, Singapore.</nlm:affiliation><country xml:lang="fr">Singapour</country><wicri:regionArea>Institute of Bioengineering and Nanotechnology, The Nanos, Singapore</wicri:regionArea></affiliation></author><author><name sortKey="Pearce, Mark C" uniqKey="Pearce M">Mark C Pearce</name></author><author><name sortKey="Ting, Boon Ping" uniqKey="Ting B">Boon Ping Ting</name></author><author><name sortKey="Ying, Jackie Y" uniqKey="Ying J">Jackie Y Ying</name></author></titleStmt><publicationStmt><date when="2011">2011</date><idno type="doi">10.1016/j.bios.2011.06.007</idno><idno type="RBID">pubmed:21782410</idno><idno type="pmid">21782410</idno><idno type="wicri:Area/Main/Corpus">001252</idno><idno type="wicri:Area/Main/Curation">001252</idno><idno type="wicri:Area/Main/Exploration">001058</idno></publicationStmt></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term><term>Biosensing Techniques</term><term>Electrochemical Techniques</term><term>Electrodes</term><term>Enzymes, Immobilized (chemistry)</term><term>Glucose Oxidase (chemistry)</term><term>Gold (chemistry)</term><term>Immunoassay</term><term>Immunoglobulin G (analysis)</term><term>Metal Nanoparticles (chemistry)</term><term>Mice</term><term>Tin Compounds</term></keywords><keywords scheme="MESH" type="chemical" qualifier="analysis" xml:lang="en"><term>Immunoglobulin G</term></keywords><keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Enzymes, Immobilized</term><term>Glucose Oxidase</term><term>Gold</term></keywords><keywords scheme="MESH" qualifier="chemistry" xml:lang="en"><term>Metal Nanoparticles</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Biosensing Techniques</term><term>Electrochemical Techniques</term><term>Electrodes</term><term>Immunoassay</term><term>Mice</term><term>Tin Compounds</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">This paper describes a novel enzymatic amplification strategy for ultrasensitive electrochemical immunosensing. This approach utilizes glucose oxidase for the enzymatic deposition of gold nanoparticles onto an indium tin oxide (ITO) electrode surface using a novel gold developer solution consisting of 20 mM of glucose, 20 mM of NaSCN, 0.5 M of p-benzoquinone (PBQ) and 1 mM of AuCl(4)(-) dissolved in 0.1 M of pH 7.5 phosphate buffer solution. The amount of gold deposited was quantified electrochemically by monitoring the reduction of gold oxide in an aqueous solution of 0.5 M of H(2)SO(4), which was correlated to the amount of antigens in the solution. The effectiveness of this strategy was demonstrated experimentally through the construction of an immunosensor for the detection of mouse IgG using a sandwich immunoassay in a linear dynamic range of 5 pg/ml to 50 ng/ml. A good mean apparent recovery in the range of 88-102% was obtained over the entire linear dynamic range of the sensor response in the serum samples. This suggested that the immunosensor would be useful for the testing of proteins in real clinical samples.</div></front></TEI><pubmed><MedlineCitation Owner="NLM" Status="MEDLINE"><PMID Version="1">21782410</PMID><DateCreated><Year>2011</Year><Month>08</Month><Day>05</Day></DateCreated><DateCompleted><Year>2011</Year><Month>12</Month><Day>07</Day></DateCompleted><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1873-4235</ISSN><JournalIssue CitedMedium="Internet"><Volume>27</Volume><Issue>1</Issue><PubDate><Year>2011</Year><Month>Sep</Month><Day>15</Day></PubDate></JournalIssue><Title>Biosensors & bioelectronics</Title><ISOAbbreviation>Biosens Bioelectron</ISOAbbreviation></Journal><ArticleTitle>Ultrasensitive electrochemical immunosensor employing glucose oxidase catalyzed deposition of gold nanoparticles for signal amplification.</ArticleTitle><Pagination><MedlinePgn>53-7</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1016/j.bios.2011.06.007</ELocationID><Abstract><AbstractText>This paper describes a novel enzymatic amplification strategy for ultrasensitive electrochemical immunosensing. This approach utilizes glucose oxidase for the enzymatic deposition of gold nanoparticles onto an indium tin oxide (ITO) electrode surface using a novel gold developer solution consisting of 20 mM of glucose, 20 mM of NaSCN, 0.5 M of p-benzoquinone (PBQ) and 1 mM of AuCl(4)(-) dissolved in 0.1 M of pH 7.5 phosphate buffer solution. The amount of gold deposited was quantified electrochemically by monitoring the reduction of gold oxide in an aqueous solution of 0.5 M of H(2)SO(4), which was correlated to the amount of antigens in the solution. The effectiveness of this strategy was demonstrated experimentally through the construction of an immunosensor for the detection of mouse IgG using a sandwich immunoassay in a linear dynamic range of 5 pg/ml to 50 ng/ml. A good mean apparent recovery in the range of 88-102% was obtained over the entire linear dynamic range of the sensor response in the serum samples. This suggested that the immunosensor would be useful for the testing of proteins in real clinical samples.</AbstractText><CopyrightInformation>Copyright © 2011 Elsevier B.V. All rights reserved.</CopyrightInformation></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Zhang</LastName><ForeName>Jie</ForeName><Initials>J</Initials><Affiliation>Institute of Bioengineering and Nanotechnology, The Nanos, Singapore, Singapore.</Affiliation></Author><Author ValidYN="Y"><LastName>Pearce</LastName><ForeName>Mark C</ForeName><Initials>MC</Initials></Author><Author ValidYN="Y"><LastName>Ting</LastName><ForeName>Boon Ping</ForeName><Initials>BP</Initials></Author><Author ValidYN="Y"><LastName>Ying</LastName><ForeName>Jackie Y</ForeName><Initials>JY</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType>Journal Article</PublicationType><PublicationType>Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2011</Year><Month>06</Month><Day>17</Day></ArticleDate></Article><MedlineJournalInfo><Country>England</Country><MedlineTA>Biosens Bioelectron</MedlineTA><NlmUniqueID>9001289</NlmUniqueID><ISSNLinking>0956-5663</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance>Enzymes, Immobilized</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance>Immunoglobulin G</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance>Tin Compounds</NameOfSubstance></Chemical><Chemical><RegistryNumber>71243-84-0</RegistryNumber><NameOfSubstance>indium tin oxide</NameOfSubstance></Chemical><Chemical><RegistryNumber>7440-57-5</RegistryNumber><NameOfSubstance>Gold</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 1.1.3.4</RegistryNumber><NameOfSubstance>Glucose Oxidase</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName MajorTopicYN="N">Animals</DescriptorName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N">Biosensing Techniques</DescriptorName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="Y">Electrochemical Techniques</DescriptorName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N">Electrodes</DescriptorName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N">Enzymes, Immobilized</DescriptorName><QualifierName MajorTopicYN="N">chemistry</QualifierName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N">Glucose Oxidase</DescriptorName><QualifierName MajorTopicYN="Y">chemistry</QualifierName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N">Gold</DescriptorName><QualifierName MajorTopicYN="Y">chemistry</QualifierName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="Y">Immunoassay</DescriptorName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N">Immunoglobulin G</DescriptorName><QualifierName MajorTopicYN="Y">analysis</QualifierName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N">Metal Nanoparticles</DescriptorName><QualifierName MajorTopicYN="Y">chemistry</QualifierName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N">Mice</DescriptorName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N">Tin Compounds</DescriptorName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2011</Year><Month>4</Month><Day>1</Day></PubMedPubDate><PubMedPubDate PubStatus="revised"><Year>2011</Year><Month>6</Month><Day>9</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2011</Year><Month>6</Month><Day>10</Day></PubMedPubDate><PubMedPubDate PubStatus="aheadofprint"><Year>2011</Year><Month>6</Month><Day>17</Day></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2011</Year><Month>7</Month><Day>26</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2011</Year><Month>7</Month><Day>26</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2011</Year><Month>12</Month><Day>13</Day><Hour>0</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pii">S0956-5663(11)00371-X</ArticleId><ArticleId IdType="doi">10.1016/j.bios.2011.06.007</ArticleId><ArticleId IdType="pubmed">21782410</ArticleId></ArticleIdList></PubmedData></pubmed></record>Pour manipuler ce document sous Unix (Dilib)
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